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	Provedor de dados J. Venom. Anim. Toxins incl. Trop. Dis. (2) BJM (1) Autor Vicentini,Adriana Pardini (3) Freitas,Roseli Santos de (2) Kohara,Valdelene Sayuri (2) Dantas,Kátia Cristina (1) Garcia,Roberta Scholz Pinto (1) Kamikawa,Camila Mika (1) Mendes,Rinaldo Poncio (1) Muricy,Edna Cleide Mendes (1) Passos,Angela Noronha (1) Silva,Marcos Vinícius da (1) Mais... Palavra-chave Immunoblotting (2) Blood fluid (1) Dot-ELISA (1) Double immunodiffusion (1) Fungal infections (1) Histoplasma (1) Histoplasmosis (1) Immunoassay (1) Immunocompetent individuals (1) Immunodiagnostic tool (1) Nested PCR (1) Paracoccidioides brasiliensis (1) Paracoccidioidomycosis (1) Validation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Info:eu-repo/semantics/report (1) Ano 2017 (1) 2014 (2) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Immunological assays employed for the elucidation of an histoplasmosis outbreak in São Paulo, SP BJM Passos,Angela Noronha; Kohara,Valdelene Sayuri; Freitas,Roseli Santos de; Vicentini,Adriana Pardini. Several reports showed outbreaks of histoplasmosis acquired while bat-inhabited caves were visited by tourists, miners or researchers. We evaluated the performance of double immunodifusion (DI) and immunoblotting (IB) assays, employed for the histoplasmosis outbreak elucidation occurred in Vale do Paraíba, São Paulo. The existence of epidemiologic link, four patients with clinical signs suggestive of histoplasmosis and mycological confirmation has made that all 35 individuals involved to the cave visit were subjected to serological evaluation. By DI, we observed reactivity against H. capsulatum antigen in a single serum examined nearly 20 days after exposure to fungal propagules. On the other hand, IB showed reactivity against H and M fractions in 50% of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Histoplasma; Histoplasmosis; Immunoassay; Double immunodiffusion; Immunoblotting. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400028 Importance of the association of molecular and immunological diagnosis in immunocompetent patient with Histoplasma capsulatum and Cryptoccocus neoformans infection: a case report J. Venom. Anim. Toxins incl. Trop. Dis. Dantas,Kátia Cristina; Freitas,Roseli Santos de; Garcia,Roberta Scholz Pinto; Silva,Marcos Vinícius da; Muricy,Edna Cleide Mendes; Kohara,Valdelene Sayuri; Vicentini,Adriana Pardini. This case reports an immunocompetent 29-year-old woman with suspected pneumonia, suggestive of fungal infection. Immunoblotting analysis reactivity againstHistoplasma capsulatum and Paracoccidioides brasiliensis were observed. Nested-PCR in blood employing species-specific primers was positive for H. capsulatum andCryptococcus neoformans. The evaluation of paucisymptomatic patients with positive results for H. capsulatum and C. neoformans could be relevant for the prevention as well as the possible evaluation of the reactivated quiescent foci. In conclusion, the associated methodology may have contributed to the monitoring endogenous reactivation of these diseases. Tipo: Info:eu-repo/semantics/report Palavras-chave: Immunocompetent individuals; Nested PCR; Immunoblotting; Fungal infections; Blood fluid. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200507 Standardization and validation of Dot-ELISA assay for Paracoccidioides brasiliensis antibody detection J. Venom. Anim. Toxins incl. Trop. Dis. Kamikawa,Camila Mika; Mendes,Rinaldo Poncio; Vicentini,Adriana Pardini. Abstract Background Paracoccidioidomycosis (PCM) is a neglected systemic mycosis caused by a dimorphic fungus of the Paracoccidioides genus. The standard diagnosis is based on isolation of the fungi in culture, and by microscopic visualization of characteristic multiple budding yeast cells in biological samples. However, in some situations, access to the site of injury prevents the collection of biological material. A variety of immuno-serological techniques has proven useful for allowing inferring diagnosis with a certain degree of certainty, thus optimizing time. The aim of this study was to standardize and validate the Dot-ELISA (DE) assay, comparing it with the serological standard, double immunodiffusion (DI). Methods In order to standardize the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Paracoccidioidomycosis; Paracoccidioides brasiliensis; Immunodiagnostic tool; Dot-ELISA; Validation. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100305 Registros recuperados: 3 Primeira ... 1 ... Última

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